Abstract
BACKGROUND: Breast cancer poses a huge health threat in China. Emerging evidence has indicated that RANBP1 is associated with poor prognosis of breast cancer and has been shown to influence miRNA expression in colorectal cancer. But its role in breast cancer remains unclear. OBJECTIVE: The purpose of this research is to construct the sh-RANBP1 cell line derived from a human breast cancer cell line in order to investigate the impact of low RANBP1 expression on the expression network of breast cancer-associated miRNAs and mRNAs. METHODS: We constructed the sh-RANBP1 cell line from the human breast cancer cell line MDA-MB-231 and analyzed the significant differential expression between the two groups using miRNA sequencing and mRNA sequencing of both the sh-RANBP1 group and the control group. The differential miRNA-mRNA regulatory network for the two groups was established by intersecting target predictions with the differential expression data. The results were further verified using Western blotting (WB), quantitative PCR (qRT-PCR), and flow cytometry. RESULTS: In this study, we compared the expression levels of miRNA and mRNA between the sh-RANBP1 group and the control group. Our findings indicate that sh-RANBP1 influences miRNA expression, revealing significant differences based on the interaction network of miRNA and mRNA. The genes involved are associated with pathways such as apoptosis. Subsequent WB and qRT-PCR results further validated our findings. Finally, flow cytometry confirmed an increased proportion of apoptosis in the sh-RANBP1 interference group. CONCLUSION: In summary, sh-RANBP1 affects miRNA expression in breast cancer cells, then regulates the expression of mRNA, and ultimately increases the proportion of apoptosis in breast cancer cells.