Comparison of matK and rbcL DNA barcodes for genetic classification of jewel orchid accessions in Vietnam

matK 和 rbcL DNA 条形码对越南宝石兰花种质进行遗传分类的比较

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作者:Viet The Ho, Thi Kim Phuong Tran, Thi Thanh Tram Vu, Sasanti Widiarsih

Background

Jewel orchid is the common name of several orchid species which can be alike in morphological characteristics, but variable in medicinal properties. At present, two DNA barcode loci, namely, maturase K (matK) and ribulose 1,5-biphosphate carboxylase (rbcL), are intensively utilized for plant identification. However, the discrimination effectiveness of these loci is variable among plant species. This study was carried out to compare the identifying efficacy of these two loci on jewel orchid population collected throughout Vietnam.

Conclusion

The findings of this project could provide valuable information that is necessary for classification, plant origin identification, breeding, and conservation program of jewel orchid in Vietnam.

Results

The results revealed that 21 jewel orchid accessions studied were segregated into four different species with significant variations. The discrimination power of matK and rbcL markers in this jewel orchid study displayed different efficiency level. The rbcL gene has higher distinguishing potential than either matK gene alone or the combination of both genes.

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