Abstract
BACKGROUND: scleroglucan, an extracellular polysaccharide with gel-forming, thickening, and stabilizing properties, was used as a structure-forming agent in low-fat yogurt formulations. The aim of this study was to evaluate its influence on the fermentation process and the physicochemical, rheological, textural, microstructural, and sensory properties of the yogurts. METHODS: control samples were formulated with the addition of skim milk powder (SMP), whereas experimental yogurts contained scleroglucan at concentrations of 0.25%, 0.5%, and 1.0% (w/w). The fermentation kinetics, acidity, color, syneresis, rheological behavior, texture profile, microstructure, and volatile compounds were analyzed during storage. RESULTS: the results showed that scleroglucan slowed acidification and increased the apparent viscosity, yield stress, and firmness of yogurts, while completely eliminating syneresis. Scleroglucan also modified the volatile profile by decreasing acetaldehyde and increasing 2,3-pentanedione levels during storage. The survival of Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus was not affected. CONCLUSIONS: the yogurt containing 1.0% scleroglucan was rated highest in overall acceptability. These findings demonstrate that scleroglucan can serve as a natural, clean-label stabilizer and an alternative to skim milk powder in low-fat set-style yogurts.