Abstract
During the present study, we re-examined species boundaries in three mountain-dwelling velvet worm species complexes (Peripatopsis balfouri s.l., P. bolandi s.l. and P. purpureus s.l.) along the Cape Fold Mountains of South Africa. We obtained DNA sequence data for both the mitochondrial cytochrome c oxidase subunit one (COI) and the nuclear 18S rRNA loci. Phylogenetic inferences were derived with the use of maximum likelihood and Bayesian inference coupled with a divergence time estimation. Four species delimitation methods (ASAP, bPTP, bGYMC and STACEY) together with gross morphological analyses and scanning electron microscopy (SEM) were used to validate the diagnosis of novel species. Combined phylogenetic results demonstrated the presence of three geographically discrete clades (A-C). Corroborative evidence for the novel lineages could be derived from the dorsal integument colour of live specimens and fixed dorsal and ventral papilla scales rank counts. The four species delimitation methods produced variable results. Divergence time estimations indicated that the Miocene epochs was a major period of cladogenesis. The seven novel velvet worm species, P. barnardi sp. nov., P. fernkloofi sp. nov., P. jonkershoeki sp. nov., P. kogelbergi sp. nov., P. landroskoppie sp. nov., P. limietbergi sp. nov., and P. palmeri sp. nov., are herein described.