Conclusion
Finally, iPS and ES cells have been directly induced to erythropoiesis without hemangioblast formation that produced CD71(+)HbF(+) erythroid cells. Although we observed some variations in the efficiency of hematopoietic differentiation between iPSC and ES cells, the pattern of differentiation was similar among all three tested lines.
Methods
In this experimental study, we examined the erythroid differentiation potential of normal Bombay hiPSCs (B-hiPSCs) and compared
Objective
There is constant difficulty in obtaining adequate supplies of blood components, as well as disappointing performance of "universal" red blood cells. Advances in somatic cell reprogramming of human-induced pluripotent stem cells (hiPSCs) have provided a valuable alternative source to differentiate into any desired cell type as a therapeutic promise to cure many human disease. Materials and
Results
Similar to hESC lines, hemangioblasts derived from B-hiPSCs expressed approximately 9% KDR(+)CD31(+) and approximately 5% CD31(+)CD34(+). In semisolid media, iPSC and hESC-derived hemangioblast formed mixed type of hematopoietic colony. In mixed colonies, erythroid progenitors were capable to express CD71(+)GPA(+)HbF(+) and accompanied by endothelial cells differentiation.