Abstract
Feed wastage in laboratory mice, also known as chewing or grinding behavior, is problematic for program management and animal welfare. The destruction of pelleted feed without consumption produces a powder accumulation on the cage floor called orts. Ort accumulation disrupts the cage microenvironment and can clog Lixits resulting in flooding. Moreover, added labor adds cost, and cage disruption increases animal stress. Published studies examining the behavior and ways to mitigate it have had inconsistent results, and the cause or causes have not yet been fully identified. The purpose of this study was to identify methods to reduce the development of chewing behavior in laboratory mice. Female Swiss Webster (Tac:SW) mice (n = 144) were randomly assigned to one of 8 groups (12 cages per group) with 2 housing densities (single and pair) and 4 nesting material paradigms. Mice were housed on clean bedding for 8 wk and then soiled bedding for the next 8 wk. Chewing behavior was evaluated by feed weight, cage weight, and feed scores. The addition of a Diamond Twist significantly increased ort production, while nest transfer decreased it but not significantly. Pair housing increased overall orts but not when adjusted for animal number. These results identified potential contributing factors to chewing behavior. However, further research is needed to elucidate the exact causes and solutions.