External quality control program for semen analysis: Spanish experience

精液分析的外部质量控制计划:西班牙的经验

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作者:C Alvarez, J A Castilla, J P Ramírez, F Vergara, A Yoldi, A Fernández, J J Gaforio

Conclusions

These data highlight the urgent need for improvement in the overall quality of andrology testing.

Methods

Quality control materials were distributed and the following seminal parameters were determined: concentration, total motility, progressive motility, rapid progressive motility, morphology and sperm vitality. The between-laboratories coefficients of variation were assessed on different types of quality control material.

Results

The majority of participating laboratories utilised manual versus computer-assisted semen analysis methods. Some between-laboratories coefficients of variation ranges were: 20.8-33.8% for concentration (semen pool suspension); 13.9-19.2% for total motility (videotapes); 54.2-70.2% for sperm morphology (strict criteria using stained smears); and 9.8-41.1% for sperm vitality (stained smears). There was an inverse relation between mean percentage of sperm and coefficients of variation between laboratories for sperm motility, morphology and vitality. Conclusions: These data highlight the urgent need for improvement in the overall quality of andrology testing.

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