Conclusions
Baotaiyin inhibits the positive feedback cycle of IL-23/Th17 immune inflammatory axis, which regulates Th17/Treg cell balance, mediates the maternal and fetal immune tolerance, and prevents the recurrent abortion. 目的: 中药治疗反复自然流产的机制尚不清楚，本研究探讨中药保胎饮通过调节白介素(interleukin，IL)-23/辅助性T细胞(helper T cell，Th)17免疫炎症轴治疗反复自然流产的作用机制。方法: 将自然流产小鼠模型随机分成模型组，保胎饮低、中、高剂量组，每组10只。用药14 d后，采用酶联免疫吸附试验(enzyme-linked immunosorbent assay，ELISA)检测血清中IL-17、IL-23、转化生长因子β(transforming growth factor，TGF-β)及IL-10的表达水平；流式细胞术检测小鼠脾淋巴细胞中Th17与调节性T细胞(regulatory T cells，Treg)的比例；RT-PCR检测蜕膜组织中维A酸相关孤儿受体γt(retinoid-related orphan receptor γt，ROR-γt)、叉头样转录因子蛋白3(forkhead box protein3，FOXP3)mRNA表达，并计算胚胎吸收率。结果: 与模型组比较，保胎饮中、高剂量组胚胎吸收率和Th17/Treg细胞比值均明显下降(均P<0.05)，血清IL-17和IL-23表达均明显降低(均P<0.05)，同时TGF-β和IL-10表达均升高(分别P<0.05和P<0.01)；蜕膜组织中RORγt mRNA表达降低而FOXP3 mRNA表达升高(均P<0.01)。结论: 保胎饮通过抑制IL-23/Th17免疫炎症轴的正反馈循环，调控Th17/Treg细胞平衡从而介导母胎免疫耐受，防止复发性流产的发生。.
Methods
Spontaneous abortion model mice were randomly divided into a model group, 3 dose (low, medium, and high) groups of baotaiyin, with 10 mice in each group. After 14 days of medication, the levels of IL-17, IL-23, IL-10, and TGF-β in serum were detected with enzyme-linked immunosorbent assay. The proportion of Th17 and regulatory T cells (Treg) cells in spleen lymphocytes was tested with flow cytometry. The expressions of (retinoid-related orphan receptor γt, ROR-γt) and forkhead box P3 (FOXP3) mRNA in decidua tissues was detected with RT-PCR. Embryo absorption rate was counted.
Results
Compared with the model group, the absorption rate of embryo and Th17/Treg cell ratio in baotaiyin medium- and high-dose groups were decreased significantly (all P<0.05); the levels of IL-17 and IL-23 in serum were decreased (both P<0.05), while the levels of TGF-β and IL-10 in baotaiyin medium- and high-dose groups were increased (P<0.05, P<0.01, respectively); the expression of ROR-γt mRNA was decreased and the expression of FOXP3 mRNA was increased (all P<0.01) in decidua tissues of baotaiyin medium- and high-dose groups. Conclusions: Baotaiyin inhibits the positive feedback cycle of IL-23/Th17 immune inflammatory axis, which regulates Th17/Treg cell balance, mediates the maternal and fetal immune tolerance, and prevents the recurrent abortion. 目的: 中药治疗反复自然流产的机制尚不清楚，本研究探讨中药保胎饮通过调节白介素(interleukin，IL)-23/辅助性T细胞(helper T cell，Th)17免疫炎症轴治疗反复自然流产的作用机制。方法: 将自然流产小鼠模型随机分成模型组，保胎饮低、中、高剂量组，每组10只。用药14 d后，采用酶联免疫吸附试验(enzyme-linked immunosorbent assay，ELISA)检测血清中IL-17、IL-23、转化生长因子β(transforming growth factor，TGF-β)及IL-10的表达水平；流式细胞术检测小鼠脾淋巴细胞中Th17与调节性T细胞(regulatory T cells，Treg)的比例；RT-PCR检测蜕膜组织中维A酸相关孤儿受体γt(retinoid-related orphan receptor γt，ROR-γt)、叉头样转录因子蛋白3(forkhead box protein3，FOXP3)mRNA表达，并计算胚胎吸收率。结果: 与模型组比较，保胎饮中、高剂量组胚胎吸收率和Th17/Treg细胞比值均明显下降(均P<0.05)，血清IL-17和IL-23表达均明显降低(均P<0.05)，同时TGF-β和IL-10表达均升高(分别P<0.05和P<0.01)；蜕膜组织中RORγt mRNA表达降低而FOXP3 mRNA表达升高(均P<0.01)。结论: 保胎饮通过抑制IL-23/Th17免疫炎症轴的正反馈循环，调控Th17/Treg细胞平衡从而介导母胎免疫耐受，防止复发性流产的发生。.