Direct application of the INNO-LiPA Rif.TB line-probe assay for rapid identification of Mycobacterium tuberculosis complex strains and detection of rifampin resistance in 360 smear-positive respiratory specimens from an area of high incidence of multidrug-resistant tuberculosis

直接应用 INNO-LiPA Rif.TB 线性探针检测法快速鉴定结核分枝杆菌复合菌株,并对来自耐多药结核病高发地区的 360 份涂片阳性呼吸道标本进行利福平耐药性检测

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作者:Miguel Viveiros, Clara Leandro, Liliana Rodrigues, Josefina Almeida, Rosário Bettencourt, Isabel Couto, Lurdes Carrilho, José Diogo, Ana Fonseca, Luís Lito, João Lopes, Teresa Pacheco, Mariana Pessanha, Judite Quirim, Luísa Sancho, Max Salfinger, Leonard Amaral

Abstract

The INNO-LiPA Rif.TB assay for the identification of Mycobacterium tuberculosis complex strains and the detection of rifampin (RIF) resistance has been evaluated with 360 smear-positive respiratory specimens from an area of high incidence of multidrug-resistant tuberculosis (MDR-TB). The sensitivity when compared to conventional identification/culture methods was 82.2%, and the specificity was 66.7%; the sensitivity and specificity were 100.0% and 96.9%, respectively, for the detection of RIF resistance. This assay has the potential to provide rapid information that is essential for the effective management of MDR-TB.

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