Abstract
The predatory stink bug, Eocanthecona furcellata, is a crucial biological control agent used globally to manage agricultural pests. Here, we present the chromosome-level genome assembly for this economically important species to provide a foundational resource for understanding its unique predatory biology. By integrating 41.94 Gb of PacBio HiFi, 56.12 Gb of Illumina, and 68.62 Gb of Hi-C sequencing data, we constructed a 1.10 Gb assembly with excellent contiguity (contig N50 = 42.08 Mb; scaffold N50 = 144.30 Mb). A total of 98.85% of the assembled sequences were anchored into eight pseudochromosomes. Genomic analysis revealed that repetitive elements comprise 41.46% of the genome, and we annotated 16,880 protein-coding genes, 98.74% of which were functionally assigned. The quality of the assembly is confirmed by its high completeness (BUSCO score = 98.8%; Merqury QV = 46.33). This high-quality genomic resource will be invaluable for future research into the molecular mechanisms of predation, insecticide resistance, and for optimizing the application of E. furcellata in integrated pest management programs.