Identification of cervical cancer stem cells using single-cell transcriptomes of normal cervix, cervical premalignant lesions, and cervical cancer

利用正常宫颈、宫颈癌前病变和宫颈癌的单细胞转录组鉴定宫颈癌干细胞

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作者:Tao Zhang, Liang Zhuang, Munawaer Muaibati, Dan Wang, Abuduyilimu Abasi, Qing Tong, Ding Ma, Lei Jin, Xiaoyuan Huang

Background

Cervical cancer is the fourth leading cause of mortality among gynecological malignancies. However, the identification of cervical cancer stem cells remains unclear.

Methods

We performed single-cell mRNA sequencing on ∼122,400 cells from 20 cervical biopsies, including 5 healthy controls, 4 high-grade intraepithelial neoplasias, 5 microinvasive carcinomas of the cervix, and 6 invasive cervical squamous carcinomas. Bioinformatic

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