Abstract
Cornus officinalis Siebold & Zucc. 1839 is a medicinally important species of Cornaceae, yet mitochondrial genomic information for this genus has remained unavailable. In this study, we assembled and annotated the mitochondrial genome of C. officinalis using PacBio HiFi sequencing data. The mitogenome comprises three circular molecules totaling 556,620 bp with similar GC contents of approximately 45%. A total of 70 genes were identified, including 43 protein-coding genes, 23 transfer RNA genes, and four ribosomal RNA genes. Phylogenetic analysis based on mitochondrial protein-coding genes placed C. officinalis in a well-supported clade with Hydrangeaceae, Ericaceae, and Primulaceae. This first mitochondrial genome reported for Cornaceae provides a useful resource for mitochondrial genome evolution and angiosperm phylogenetic studies.