Abstract
Salmonella enterica subsp. enterica serovar Enteritidis is a well-known non-typhoidal serovar, commonly associated with foodborne illnesses. Here, we report the draft genome sequence of Salmonella enterica subsp. enterica serovar Enteritidis strain SSTRA25, isolated from a pediatric patient with bacteremia in Mosul, Iraq. The genome was sequenced using the Illumina NovaSeq 6000 platform. The assembled and annotated genome comprised 4733,231 bp with 40 contigs, and a GC content of 52.12%. It contains 4580 coding sequences (CDSs), 69 tRNAs, 9 rRNAs, 14 ncRNAs, 2 CRISPR arrays, and 368 annotated subsystems. The analysis of antimicrobial resistance genes revealed multiple genes associated with various drug classes, including phenicols, penicillin beta-lactams, cephalosporins, carbapenems, and monobactams, with perfect sequence matches. In addition, chromosomal point mutations linked to antimicrobial resistance were identified with significant sequence similarity. Salmonella enterica subsp. enterica serovar Enteritidis SSTRA25 showed a high predicted human pathogenicity score (0.941) and carried multiple virulence factors, including SspH2, SopA, SadA, ShdA, MisL, and several flagellar and outer membrane proteins. In the pathogenic landscape, the closest strain was Salmonella enterica subsp. enterica serovar Holcomb NY_FSL C7-1028, with a Minkowski distance of 0.024804. The genome sequence of Salmonella enterica subsp. enterica serovar Enteritidis SSTRA25 has been deposited in NCBI under the accession number JBNHMR000000000.