Complex CatSper-dependent and independent [Ca2+]i signalling in human spermatozoa induced by follicular fluid

卵泡液诱导的人类精子中复杂的 CatSper 依赖性和独立的 [Ca2+]i 信号传导

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作者：Sean G Brown, Sarah Costello, Mark C Kelly, Mythili Ramalingam, Ellen Drew, Stephen J Publicover, Christopher L R Barratt, Sarah Martins Da Silva

期刊：

Human Reproduction

影响因子：

6.000

时间：

2017

起止号：

2017 Oct 1;32(10):1995-2006.

doi：

10.1093/humrep/dex269

研究方向：

信号转导

Methods

Semen donors and patients were recruited in accordance with local ethics approval (13/ES/0091) from the East of Scotland Research Ethics Service REC1. Activities of CatSper and KSper were assessed by patch clamp electrophysiology. Sperm [Ca2+]i responses were examined in sperm populations and single cells. Computer-assisted sperm analysis (CASA) parameters and penetration into viscous media were used to assess functional effects. Main

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