Effect of maternal obesity on estrous cyclicity, embryo development and blastocyst gene expression in a mouse model

母体肥胖对小鼠发情周期、胚胎发育和囊胚基因表达的影响

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作者:Pablo Bermejo-Alvarez, Cheryl S Rosenfeld, R Michael Roberts

Methods

Females were fed either a control diet (C, n = 9) or a diet high in fat [diet-induced obesity (DiO), n = 12] for 12 weeks, and were then co-housed with fertile males. Mice that failed to breed during 20 consecutive days were considered infertile. Control and diet-induced obese females that demonstrated vaginal plugs were euthanized 3.5 days after mating, blood was sampled for glucose and hormone measurements, corpora lutea counted and embryos recovered; the relative mRNA abundance of 11 candidate genes was determined in blastocysts by qPCR. Main

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