Comparison of loop-mediated isothermal amplification and conventional PCR tests for diagnosis of common Brucella species

Rapid, reliable, and affordable detection of Brucella species via the molecular

A dilution series of B. abortus DNA indicated that the LAMP reaction could reliably detect 10 (fg/µl) DNA target copies per reaction within 36 min, which is 10 times greater than the PCR assay. In summary, we conclude that LAMP assay provide accurate and fast test results to identify of common Brucella species in low-complexity labs, mainly in low and lower middle income countries.