Abstract
Polyherbal formulations (PHFs) serve as a cornerstone of traditional medical systems worldwide and remain integral to diverse therapeutic practices. The COVID-19 pandemic has reinvigorated scientific interest in PHFs, driving extensive investigations into their composition and pharmacological potential. In this study, we systematically analyzed PHFs across major traditional medical systems, including Traditional Chinese Medicine (TCM), Kampo, Ayurveda, and Unani. To facilitate comprehensive analysis, we developed the Global Polyherbal Formulation Database (GPFD), a repository documenting formulation names, sources, historical evolution, ingredient composition, dosages, medicinal origins, and therapeutic applications. Leveraging this extensive dataset, we conducted a cross-system comparative analysis of PHFs using statistical analysis and data integration approaches. Our investigation focused on plant usage frequency and co-occurrence patterns to uncover underlying principles of formulation design. This study establishes a research framework for PHFs, explores the relationship between species selection, and ecological distributions, and identifies key taxonomic differences in plant utilization across systems. Additionally, we reveal distinct preferences in herbal combinations within each system, highlighting both shared and unique formulation patterns. These findings provide critical insights into the cross-cultural utilization of PHFs and offer a data-driven foundation for integrating traditional botanical knowledge into modern pharmacological research and drug development.