First report and new molecular and morphological characterizations of root-knot nematode, Meloidogyne javanica, infecting ginger and long coriander in Vietnam

首次报道越南生姜和芫荽根结线虫(Meloidogyne javanica)的感染情况,并对其进行了新的分子和形态学表征

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Abstract

Ginger (Zingiber officinale Roscoe) and long coriander (Eryngium foetidum L.) are commonly grown and used as important spices and medicinal plants in Vietnam. Our study recovered for the first time one of the most damaging tropical root-knot nematodes, Meloidogyne javanica, associated with these plants in the Western Highlands of Vietnam. In this study, M. javanica was characterized based on morphology and molecular characterization of D2-D3 fragment of 28S rRNA, ITS, and Nad5 mtDNA regions. The identification of this species was done based on a combination of morphology, multiplex-PCR with specific primer, network haplotype analysis, and PPNID program.

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