Abstract
Two new species, Anastrephadorsidentata Uramoto, Zucchi, Araújo & Savaris and A.norrbomi Uramoto, Zucchi, Araújo & Savaris, from a fragment of the Amazon Rainforest in the city of Manaus, state of Amazonas, Brazil, are described and illustrated. Both species were collected in McPhail-type traps hanging from trees on the campus of Federal University of Amazon. A pictorial key to 47 species of Anastrepha recorded in Amazonas state is provided.