Abstract
N(6)-methyladenosine (m(6)A) RNA modification plays important regulatory roles in plant development and adaptation to the environment. However, there has been no research regarding m(6)A RNA methyltransferases (MT-A70) in Przewalskia tangutica Maxim. Here, we performed a comprehensive analysis of the MT-A70 family in Przewalskia tangutica (PtMTs), including gene structures, phylogenetic relationships, conserved motifs, gene location, promoter analysis, GO enrichment analysis, and expression profiles. We identified seven PtMT genes. Phylogeny analysis indicated that the seven PtMT genes could be divided into three groups; two MTA genes, three MTB genes, and two MTC genes, and domains and motifs exhibited similar patterns within the same group. These PtMT genes were found to contain a large number of cis-acting elements associated with plant hormones, light response, and stress response, suggesting their widespread regulatory function. Furthermore, the expression profiling of different tissues was investigated using RNA-seq data, and the expression of seven genes was further validated by qPCR analysis. These results provided valuable information to further elucidate the function of m(6)A regulatory genes and their epigenetic regulatory mechanisms in Przewalskia tangutica.