Abstract
This study aimed to reveal the landfill bacterium Priestia sp. isolate TL1 as a PHA-producing bacterium via PHA quantification and gene annotation studies. Screening of landfill isolates identified Priestia sp. isolate TL1 as a promising PHA producer. Evaluation on the effect of different glucose concentrations (1–5%) on PHA accumulation revealed that optimal production was observed at 3% of glucose concentration, while excessive glucose levels negatively affected PHA yield. Statistical analysis indicated no significant association between cell dry weight and PHA yield (p > 0.05), suggesting that biomass accumulation does not directly predict PHA production efficiency. Whole-genome sequencing performed on isolate TL1 confirmed the taxonomic identity of TL1 as Priestia sp. Gene annotation identified key genes associated with PHA biosynthesis, which included phaC, encoding a Class IV PHA synthase. This suggested that PHA production by isolate TL1 may be restricted to short-chain-length PHAs. These findings highlight the potential of Priestia sp. (isolate TL1) for cost-effective PHA production for sustainable bioplastic development. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s42770-025-01857-w.