Abstract
The Notch signaling pathway is an important conserved pathway for normal homeostasis during development. However, targeted deletion of Notch4 (Notch4(d1) ) or Notch3 (Notch3(d1) ) in mice is not lethal. In fact, both Notch4(d1) and Notch3(d1) mice develop normally and are fertile. Here we present RNA seq analysis of differential gene expression in the kidneys of Notch4(d1) mice versus the Notch3 (d1) mice, all on FVB background. Kidneys were collected from Notch4(d1) and Notch3 (d1) littermates at 3 months of age. RNA sequencing was carried out. The raw data were analyzed for differential gene expression using a negative binomial generalized linear model in the DeSeq2 software package. We used P-value ≤0.05 and an absolute fold change of 1.5 or greater to identify top upregulated and downregulated genes in Notch4 (d1) mice compared to Notch3 (d1) mice. The data provided will indentify targets of Notch3 and Notch4 signaling, specifically in kidney diseases where Notch3 or Notch4 are abberantly or redundantly expressed.