Abstract
The data provided in this article are related to research entitled "Efficiency of graphene oxide nanoparticles as delivery system of SOX2OT siRNA". In this research, the goal is to use PEI-functionalized graphene oxide (PEI-GO) as a carrier for SOX2OTsiRNA delivery. In this article describes how GO coated with PEI and it was tested whether it can be siRNA carrier in NTERA2? Can it absorb siRNA? Whether Go-PEI affects the viability of NTERA2 (NT2: human embryonic carcinoma stem cell), and HeLa cell lines. In this experiment, graphene oxide nanoparticles functionalized with a polycationic polymer, polyethylenimine (PEI). GO-PEI formation was verified with DLS, FTIR tests and zeta sizer. siRNA absorption ability of GO-PEI was tested by gel retardation assay in various weight ratios of GOPEI/siRNA (GOPEI weight/siRNA weight) (w/w ratio). The cell lines were treated with different concentrations of GO-PEI nanoparticles for 24 and 48 hours. Also, the NT2 cells were treated with different concentrations of GO-PEI nanoparticles and PEI for 36 hours. Cytotoxicity of GO-PEI were investigated by calculating the percent of cell survival by MTT assay. MTT data analyzed in excel. Researchers, who want to research on different drugs, could transfer the drug to NT2, HeLa and other cancer cells on GO-PEI (concentration 0 up to 100 mg/L).