[Expression of connexin 43 in peripheral blood monocytes from patients with acute coronary syndrome]

Patients with UAP and AMI have activated inflammatory responses and reverse changes in Cx43 expression in the PBMCs, suggesting the different roles of Cx43 in the pathogenic mechanisms of different types of ACS. 目的: 研究急性冠脉综合征(ACS)患者外周血单核细胞(PBMCs)上缝隙连接蛋白(Cx) 43的表达变化及意义。 方法: 选取2018年1月~2018年6月期间入住我科的初诊ACS患者40例, 其中不稳定型心绞痛(UAP)患者20例, 急性心肌梗死(AMI)患者20例, 选取20例同期健康体检者作为对照组。采集所有受试者外周静脉血, 分别提取血浆和单核细胞, 采用酶联免疫吸附试验(ELISA)和免疫比浊法(TIIA)检测血浆中白细胞介素(IL) -1β和高敏C-反应蛋白(hs-CRP)含量, 运用荧光定量逆转录聚合酶链反应(RT-PCR)和Western blot法检测PBMCs中Cx43 mRNA和蛋白的表达水平。 结果: 与对照组相比, UAP组患者外周血浆中IL-1β及hs-CRP的含量均有明显升高(P < 0.001), PBMCs中Cx43 mRNA和蛋白的表达量也显著增高(P < 0.001); AMI组患者外周血浆中IL-1β及hs-CRP的含量较UAP组进一步升高(P < 0.001及P < 0.01);而AMI组患者PBMCs中Cx43 mRNA和蛋白的表达量却较UAP组和对照组均有明显降低(P < 0.05)。 结论: UAP和AMI患者体内存在不同程度的炎症激活, 且Cx43在两者PBMCs中的表达量呈现相反的表达变化, 提示Cx43可能在ACS不同类型发病中起到不同效应。

We prospectively collected the clinical data from 40 patients with ACS including 20 with unstable angina pectoris (UAP) and 20 with acute myocardial infarction (AMI) admitted in our department between January, 2018 and June, 2018, with 20 healthy subjects undergoing routine physical examinations serving as the control group. Peripheral blood samples were obtained from all the participants and plasma and PBMCs were separated. Enzyme-linked immunosorbent assay (ELISA) and turbidimetric inhibition immunoassay (TIIA) were used for analysis of plasma levels of interleukin (IL)-1β and high sensitive C-reactive protein (hs-CRP), respectively; real-time quantitative RT-PCR and Western blotting were used to detect the mRNA and protein levels of Cx43 in the PBMCs.

To investigate the expression of connexin 43 (Cx43) in peripheral blood monocytes (PBMCs) from patients with acute coronary syndrome (ACS) and its clinical implications.

Compared with the control group, the patients with UAP showed significantly increased plasma levels of IL-1β and hs-CRP (P < 0.001) and obviously elevated expressions of Cx43 at both mRNA and protein levels in the PBMCs (P < 0.001). Compared with the patients with UAP, the patients with AMI had significantly higher plasma IL-1β and hs-CRP levels (P < 0.001 and P < 0.01) but lower expression levels of Cx43 in the PBMCs (P < 0.05). Conclusions: Patients with UAP and AMI have activated inflammatory responses and reverse changes in Cx43 expression in the PBMCs, suggesting the different roles of Cx43 in the pathogenic mechanisms of different types of ACS. 目的: 研究急性冠脉综合征(ACS)患者外周血单核细胞(PBMCs)上缝隙连接蛋白(Cx) 43的表达变化及意义。 方法: 选取2018年1月~2018年6月期间入住我科的初诊ACS患者40例, 其中不稳定型心绞痛(UAP)患者20例, 急性心肌梗死(AMI)患者20例, 选取20例同期健康体检者作为对照组。采集所有受试者外周静脉血, 分别提取血浆和单核细胞, 采用酶联免疫吸附试验(ELISA)和免疫比浊法(TIIA)检测血浆中白细胞介素(IL) -1β和高敏C-反应蛋白(hs-CRP)含量, 运用荧光定量逆转录聚合酶链反应(RT-PCR)和Western blot法检测PBMCs中Cx43 mRNA和蛋白的表达水平。 结果: 与对照组相比, UAP组患者外周血浆中IL-1β及hs-CRP的含量均有明显升高(P < 0.001), PBMCs中Cx43 mRNA和蛋白的表达量也显著增高(P < 0.001); AMI组患者外周血浆中IL-1β及hs-CRP的含量较UAP组进一步升高(P < 0.001及P < 0.01);而AMI组患者PBMCs中Cx43 mRNA和蛋白的表达量却较UAP组和对照组均有明显降低(P < 0.05)。 结论: UAP和AMI患者体内存在不同程度的炎症激活, 且Cx43在两者PBMCs中的表达量呈现相反的表达变化, 提示Cx43可能在ACS不同类型发病中起到不同效应。