Abstract
A Co MOF (Co-2MI) nanozyme with uniform spherical nanoflower morphology was constructed with efficient laccase-like activity. Co-2MI displayed lower K (m) and higher V (max) than natural Laccase, indicating stronger substrate affinity and superior catalytic efficiency. Mechanistic investigations confirmed an ˙O(2) (-)-mediated oxidation pathway. Based on its laccase-like activity, we established a novel colorimetric method for Salbutamol detection, obtaining a linear range of 1-60 μg mL(-1) and a low detection limit of 0.91 μg mL(-1). Finally, the Co-2MI nanozyme was successfully applied to the colorimetric detection of Salbutamol in real samples.