Abstract
In the current study, the potential interaction mechanisms between capmatinib (CAP), a selective tyrosine kinase inhibitor, and calf thymus double-stranded DNA (ds-DNA) were evaluated. In this research, we construct an amplified electrochemical platform based on a disposable pencil graphite electrode (PGE) modified with nanostructured CeO(2) decorated carbon nanofiber ceramic film (CeNPs@CNF-CF) for monitoring CAP-dsDNA interaction at physiological pH. The morphology and structure of the obtained CeNPs@CNF nanocomposite were characterized. The CeNPs@CNF-CF/PGE was characterized by scanning electron microscopy (SEM). The CAP-dsDNA interaction was examined using cyclic voltammetry (CV) and square wave voltammetry (SWV) techniques. Voltammetric experiments were conducted using CeNPs@CNF-CF/PGE. The interaction of CAP with dsDNA was investigated after applying different incubation times. The addition of dsDNA to the CAP solution decreased the peak currents of the latter and led to a negative shift in peak potentials, suggesting that the electrostatic type of interaction is the most likely to occur. SWV was employed to quantify dsDNA, demonstrating excellent sensitivity (LOD = 5 × 10(-8) M). The binding constant (K (b)) of CAP and dsDNA was calculated to be 4.54 ± 0.18 × 10(4) M(-1) using SW voltammetric data.