Statistical connection of binomial photon counting and photon averaging in high dynamic range beam-scanning microscopy

高动态范围光束扫描显微镜中二项式光子计数和光子平均的统计联系

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作者:Ryan D Muir, David J Kissick, Garth J Simpson

Abstract

Data from photomultiplier tubes are typically analyzed using either counting or averaging techniques, which are most accurate in the dim and bright signal limits, respectively. A statistical means of adjoining these two techniques is presented by recovering the Poisson parameter from averaged data and relating it to the statistics of binomial counting from Kissick et al. [Anal. Chem. 82, 10129 (2010)]. The point at which binomial photon counting and averaging have equal signal to noise ratios is derived. Adjoining these two techniques generates signal to noise ratios at 87% to approaching 100% of theoretical maximum across the full dynamic range of the photomultiplier tube used. The technique is demonstrated in a second harmonic generation microscope.

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