Abstract
Drosophila suzukii, an invasive pest native to East Asia, has spread globally over the past two decades, posing severe threats to soft-skinned fruits due to its strong reproductive capabilities and high adaptation. However, the absence of high-quality genomic resources has hindered a comprehensive understanding of its ecological adaptations and invasion strategies. Here, we present a chromosome-level genome assembly of D. suzukii using PacBio HiFi, Illumina short, and Hi-C data. The assembled genome size totals 157.35 Mb with a scaffold N50 of 25.66 Mb by anchoring 86.59% (136.24 Mb) of the assembly to six chromosomes. BUSCO analysis indicates completeness, with a score of 98.1% (n = 1,367), comprising 97.4% single-copy BUSCOs and 0.7% duplicated BUSCOs. The genome contains 17.41% (27.39 Mb) repetitive elements and encodes 14,742 predicted protein-coding genes. Comparative genomic analyses reveal strong chromosomal synteny between D. suzukii and Drosophila melanogaster. This high-quality genome assembly is a critical resource for elucidating the evolutionary dynamics, ecological adaptations, and invasive biology of D. suzukii, providing valuable insights into pest control strategies.