Abstract
'Huizao' is a leading jujube (Ziziphus jujuba Mill.) variety valued for its high-quality dry fruit. Using PacBio HiFi long reads and Hi-C data, we generated a high-quality, chromosome-level, haplotype-resolved genome assembly for this cultivar, with genome sizes of 371.22 Mb and 385.42 Mb for the two haplotypes, and corresponding N50 values of 30.69 Mb and 31.26 Mb. Over 99.9% of the assembled sequences were anchored to 12 chromosomes. Genome annotation identified 32,065 protein-coding genes in Hap1 and 33,004 in Hap2, with 29,874 allelic gene pairs supported by collinearity and sequence similarity. Comparative analyses revealed extensive structural variants and allelic differences between the two haplotypes. This high-quality assembly addresses a critical gap in genomic resources for the 'Huizao' cultivar and provides a valuable foundation for allele-aware analyses, molecular breeding, and genetic diversity research in jujube.