Stachys Species: Comparative Evaluation of Phenolic Profile and Antimicrobial and Antioxidant Potential

水苏属植物:酚类成分和抗菌及抗氧化潜力的比较评价

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作者:Daniela Benedec, Ilioara Oniga, Daniela Hanganu, Brîndușa Tiperciuc, Adriana Nistor, Ana-Maria Vlase, Laurian Vlase, Cristina Pușcaș, Mihaela Duma, Cristian Cezar Login, Mihaela Niculae, Radu Silaghi-Dumitrescu

Methods

DPPH, FRAP, nitrite-induced autooxidation of hemoglobin, inhibition of cytochrome c-catalyzed lipid peroxidation, and electron paramagnetic resonance spectroscopy. The in vitro antimicrobial properties were assessed using agar-well diffusion, broth microdilution, and antibiofilm assays. Fifteen polyphenols were identified using LC-MS and chlorogenic acid was the major component in all the samples (1131.8-6761.4 μg/g). S. germanica, S. palustris, and S. byzantina extracts each displayed an intense antiradical action in relation to high contents of TPC (6.40 mg GAE/mL), flavonoids (3.90 mg RE/mL), and caffeic acid derivatives (0.89 mg CAE/mL). In vitro antimicrobial and antibiofilm properties were exhibited towards Candida albicans, Gram-positive and Gram-negative strains, with the most intense efficacy recorded for S. germanica and S. byzantina when tested against S. aureus. These

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