Methods
We assessed the osteogenic activity of MMBP by molecular docking, MC3T3-E1 cell proliferation assay and zebrafish growth model, and evaluated its effect on osteoporosis (OP) using a retinoic acid-induced osteoporosis rat model.
Results
Sciaena ossificans bone is rich in nutrients, including 11.40% water, 59.30% ash, 1.60% crude fat, 27.10% crude protein, and 0.58% total sugars. The total amino acids account for 22.13%, including 4.33% essential amino acids and 17.80% non-essential amino acids. The mineral content was rich, with calcium, phosphorus and selenium contents of 162511, 7151, and 0.264 mg/kg, respectively. MMBP significantly promoted the proliferation of MC3T3-E1 cells, facilitated the growth and bone development of zebrafish. In retinoic acid-induced osteoporosis rat model, increased the serum calcium and phosphorus levels, attenuated the calcium loss, and reduced the tartrate-resistant acid phosphatase and alkaline phosphatase (ALP) activities and significantly improved bone density. MMBP shows potential as a functional food ingredient due to its osteogenic properties, which may help promote bone growth and maintain bone health. These findings provide a scientific basis for the high-value utilization of Miichthys miiuy by-products and a new direction for the development of novel functional food ingredients.