Abstract
The two-spot cotton leafhopper, Amrasca biguttula (Ishida) (Hemiptera: Cicadellidae), was recently detected in Florida and other southeastern states, USA. This is a quarantine pest of regulatory significance, since it can infest staple crops, such as okra, cotton, eggplant, and tropical hibiscus. While collecting infested okra plants in Homestead, Florida, five female Anagrus (Hymenoptera: Mymaridae) parasitoids emerged from eggs of Am. biguttula. The specimens were identified morphologically and molecularly by sequencing the cytochrome c oxidase subunit I (COI) gene and internal transcribed spacer 2 (ITS2) region of nuclear ribosomal RNA. Two Anagrus species were identified: Anagrus vulneratus and Anagrus sp. near vulneratus. These parasitoids are not known to occur in the Old World, the origin of Am. biguttula. Rather, they are native to North America. The available evidence suggests that the collected specimens switched from unknown local hosts in southern Florida to parasitize eggs of the invasive Am. biguttula. Future research to ascertain the identity of A. sp. near vulneratus and evaluate the efficiency of both parasitoids as natural enemies of Am. biguttula is warranted.