Abstract
Enterococcus faecium T-110 (EFT110), Bacillus subtilis TO-A (BSTOA), and Clostridium butyricum TO-A (CBTOA) are probiotic bacteria used as supplements to improve intestinal symptoms in humans, chickens, pigs, cattle, and fish. However, their effects on hosts remain unclear. Our previous study showed that EFT110, BSTOA, and CBTOA extend the lifespan of Caenorhabditis elegans . In this study, we investigated the effects of these three strains on fat accumulation in nematodes; and observed that each bacterium significantly reduced fat accumulation by regulating gene expression in lipid metabolic pathways.