Improvement of image resolution by combining enhanced confocal microscopy and quantum dot triexciton imaging

通过结合增强共聚焦显微镜和量子点三激子成像来提高图像分辨率

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作者:Simon Hennig, Dietmar J Manstein

Abstract

Super-resolution fluorescence imaging provides critically improved information about the composition, organization, and dynamics of subcellular structures. Quantum dot triexciton imaging (QDTI) has been introduced as an easy-to-use sub-diffraction imaging method that achieves an almost 2-fold improvement in resolution when used with conventional confocal microscopes. Here, we report an overall 3-fold increase in lateral and axial resolution compared to conventional confocal microscopes by combining QDTI with state-of-the-art commercial laser scanning microscope systems.

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