Polyethyleneimine-based transient gene expression processes for suspension-adapted HEK-293E and CHO-DG44 cells
基于聚乙烯亚胺的悬浮适应 HEK-293E 和 CHO-DG44 细胞瞬时基因表达过程
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作者：David L Hacker, Divor Kiseljak, Yashas Rajendra, Sarah Thurnheer, Lucia Baldi, Florian M Wurm
Abstract
Transient gene expression (TGE) from mammalian cells is an increasingly important tool for the rapid production of recombinant proteins for research applications in biochemistry, structural biology, and biomedicine. Here we review methods for the transfection of human embryo kidney (HEK-293) and Chinese hamster ovary (CHO) cells in suspension culture using the cationic polymer polyethylenimine (PEI) for gene delivery.
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