Abstract
Cyclase-associated actin cytoskeleton regulatory protein 2 (CAP2) is a conserved actin-binding protein that promotes actin filament (F-actin) turnover by disassembling ADF/cofilin-decorated filaments, supporting F-actin remodeling in differentiating cells and tissues. In the ocular lens, the actin cytoskeleton is critical for maintaining tissue biomechanical properties during fiber cell maturation. To assess CAP2's role in the lens, we examined lens-specific CAP2 knockout (CAP2 (cKO) ) mice at cellular and tissue levels. CAP2 (cKO) lenses were normal in size, shape, and transparency but exhibited increased stiffness under compression and enhanced recovery after load removal. While total actin levels and F-actin were unchanged, immunofluorescence revealed higher levels of Tropomodulin 1 (Tmod1, F-actin pointed-end capping), Tropomyosin3.5 (Tpm3.5, F-actin stabilizing), and T-plastin (F-actin bundling) in F-actin-rich membrane protrusions of mature fibers, while α-actinin-1 (F-actin cross-linking) was reduced. These findings suggest that CAP2 loss disrupts F-actin remodeling, promoting filament stabilization through Tpm3.5 binding, Tmod1 capping and T-plastin bundling. Consequently, F-actin networks become stiffer and more resilient, altering lens biomechanics. This study provides the first evidence that CAP2 regulates cell biomechanical properties in a non-muscle tissue through modulation of F-actin-associated proteins.