Abstract
Soybean aphid (Aphis glycines) is one of the main pests on soybeans, which causes serious damage to the soybean worldwide. The current genome of the soybean aphid is quite fragmented, which has impeded scientific research to some extent. In this study, we assembled a chromosome-level genome of the soybean aphid using MGI short reads, PacBio HiFi long reads and Hi-C reads. The genome sequence was anchored to four pseudo-chromosomes, with a total genome length of 324 Mb and a scaffold N50 length of 88.85 Mb. We evaluated the genome based on insecta_odb10 and the results show it has a completeness of 97.2%. A total of 20,781 protein-coding genes were predicted in the genome, of which 17,183 genes were annotated in at least one protein database. Our work provides a new genomic resource for the soybean aphid study.