Abstract
Ormosia henryi Prain, belonging to the Fabaceae family, is an endangered tree species with great economic, ornamental, and medicinal potential. The lack of genetic information and high-quality genome assembly hinders in-depth research on this species as well as its conservation and further utilization. Here, we assembled a chromosome-level genome of O. henryi using Pac-Bio HiFi, Hi-C and short read sequencing. The assembled genome size is approximately 2.69 Gb, with a scaffold N50 of 354.08 Mb and a complete BUSCO score of 98.20%. About 99.97% of the assembled sequences could be anchored to eight pseudo-chromosomes, and 69.09% of the assembled genome were repetitive sequences. In total, we predicted 42,260 protein-coding genes, 88.42% of which could be functionally annotated. This genome assembly provides a valuable resource for various applications, including future conservation and utilization, evolutionary studies, and elucidation of pathways involved in key biological traits of O. henryi.