Abstract
Bibio Geoffroy, 1762 is a species-rich genus within the family Bibionidae. In this study, we present the complete mitochondrial genome of Bibio hordeiphagus Yang and Luo 1988 for the first time. Complete mitochondrial genome of B. hordeiphagus is 15,496 bp in length, comprising 13 protein-coding genes (PCGs), 22 tRNA genes, two rRNA genes, and a control region. The nucleotide composition of mitochondrial genome of B. hordeiphagus was 40.55% of A, 39.02% of T, 8.37% of G, and 12.05% of C. Based on the sequencing and annotating results, we conducted a preliminary phylogenetic analysis of Bibionomorpha. Our findings support the monophyly of both family Bibionidae and genus Bibio, with the family Bibionidae identified as the sister group to Sciaridae and Mycetophilidae. This study encompasses all reliable annotated mitochondrial genome data for Bibionidae and analyzed the higher-level phylogenetic relationships within the Bibionomorpha from the perspective of mitochondrial genome data.