Abstract
Trichogramma japonicum is one of the successfully industrialized Trichogramma species in China and holds significant application value for controlling the rice pests Chilo suppressalis. However, progress in exploring its gene functions to enhance parasitic efficiency has been hindered by the lack of a known genomic landscape. In this study, we assembled a chromosome-level genome of T. japonicum by combining HiFi sequencing and Hi-C technology. The assembled genome is 216.96 Mb, with a scaffold N50 of 40.53 Mb, and 98.09% of contigs were anchored to five chromosomes. The final genome assembly showed a high level of completeness with a 97.44% score according to BUSCO analysis results. Genome annotation revealed that repetitive sequences constitute 26.45% of the assembly and identified 16,894 protein-coding genes. This high-quality genome assembly of T. japonicum will serve as a genomic resource for comparative genomic analysis of Trichogramma species and provide a molecular foundation and framework for understanding the interaction mechanisms between host and parasitoids.