Abstract
Halichoeres margaritaceus Valenciennes 1839 is a polygynous species of coral fish. We sequenced the complete mitochondrial genome of H. margaritaceus, and conducted a comprehensive analysis to reveal its characteristics. Results showed that the mitochondrial genome is a closed circular molecule comprising 16,710 bp. Phylogenetic analysis based on mitochondrial whole genome sequences revealed H. margaritaceus as a sister group of H. ornatissimus, H. tenuispinis, etc., with maximal support. This study presents, for the first time, the complete mitochondrial genome of H. margaritaceus, offering valuable insights that can aid in future research on the biodiversity and conservation management of this species.