Abstract
Cyperus iria is an aggressive weed of rice throughout the world. Until now, the reference genome of C. iria has not been published. Here, we completed the chromosome-level genome assembly of C. iria based on Illumina, PacBio and Hi-C reads. The assembled genome size of C. iria was 479.08 Mb with a contig N50 of 7.02 Mb. 68 pseudochromosomes were produced using Hi-C scaffolding, accounting for 99.65% of the assembled genome. The number of predicted protein-coding genes is 47,395, of which 93.26% were annotated, and 37.69% repetitive sequences were identified. Our study provided a valuable genomic resource for the molecular biology research and the management of C. iria.