Abstract
Lepturichthys fimbriata is a small benthic fish inhabiting rapid rocky shoals within the Jinsha River system. This species holds considerable significance for studying animal morphology, ecology, and evolution due to its specialized habitat adaptations. We generated the first chromosome-level genome assembly of L. fimbriata through integrated HiFi, Illumina, and Hi-C sequencing. The 591 Mb genome features a scaffold N50 of 20.9 Mb, with 94.2% (556.18 Mb) of sequences anchored to 25 pseudo-chromosomes (2n = 50). Genome annotation identified 36.01% (212.72 Mb) repetitive elements and 21,320 protein-coding genes, with assembly completeness evidenced by a 97.3% BUSCO score. This high-quality genome provides a crucial resource for investigating L. fimbriata genetic diversity and ecological adaptation mechanisms.