Abstract
Arundina graminifolia is a popular terrestrial orchid in Southeast Asia. It has high medicinal and ornamental value. In this study, the chloroplast genome of A. graminifolia was determined from BGISEQ-500 sequencing data. The total chloroplast genome was 159,482 bp in length, consisting of a large single-copy region (LSC 87,285 bp), a small single-copy region (SSC 18,581 bp), and two inverted repeat regions (IRA and IRB 26,813 bp). The complete chloroplast genome contains 135 genes, including 88 protein-coding genes, 38 transfer RNA (tRNA) genes, and 8 ribosomal RNA (rRNA) genes. In addition, the phylogenetic analysis indicates that A. graminifolia was sister to Bletilla ochracea, Bletilla striata, and Neottia fugongensis. The chloroplast genome will contribute to the research and conservation of A. graminifolia.