Abstract
The complete mitochondrial genome of Liobagrus styani was sequenced by the long and accurate polymerase chain reaction and primer walking sequence method, and each partition was characterized. This genome, with 16,515 bp in length, includes 13 protein-coding genes, 22 tRNA genes, 2rRNA genes, and 2 non-coding regions. Genes encoding on the genome are similar among all vertebrates. These genes except ND6 and 8 tRNA genes were encoded on the H-strand. Phylogenetic relationship of this species with other congeners was inferred using Bayesian Inference methods based on the genome. Result is contrast to traditional biogeographic explanation for faunal similarity and highlight the need for a further investigation on the formation of allopatric distribution pattern of the genus Liobagrus in East Asia.