Open-source microscope add-on for structured illumination microscopy

用于结构照明显微镜的开源显微镜附加组件

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作者:Mélanie T M Hannebelle #, Esther Raeth #, Samuel M Leitao, Tomáš Lukeš, Jakub Pospíšil, Chiara Toniolo, Olivier F Venzin, Antonius Chrisnandy, Prabhu P Swain, Nathan Ronceray, Matthias P Lütolf, Andrew C Oates, Guy M Hagen, Theo Lasser, Aleksandra Radenovic, John D McKinney, Georg E Fantner

Abstract

Super-resolution techniques expand the abilities of researchers who have the knowledge and resources to either build or purchase a system. This excludes the part of the research community without these capabilities. Here we introduce the openSIM add-on to upgrade existing optical microscopes to Structured Illumination super-resolution Microscopes (SIM). The openSIM is an open-hardware system, designed and documented to be easily duplicated by other laboratories, making super-resolution modality accessible to facilitate innovative research. The add-on approach gives a performance improvement for pre-existing lab equipment without the need to build a completely new system.

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