Molecular typing of nosocomial strains of Legionella pneumophila by arbitrarily primed PCR

利用任意引物PCR对医院内感染的嗜肺军团菌菌株进行分子分型

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Abstract

Arbitrarily primed PCR with two different primers was compared with ribotyping and monoclonal antibody analysis for typing Legionella strains. Applied to 11 epidemiologically unrelated strains, arbitrarily primed PCR resulted in an index of discrimination of 100% with both primers. It was found able to identify an epidemic clone of Legionella pneumophila serogroup 1 that was isolated from both patients and a hot water circuit of the same hospital.

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