Transcriptome sequencing and molecular markers discovery in the gonads of Portunus sanguinolentus

血红梭子蟹生殖腺的转录组测序和分子标记发现

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作者:Yin Zhang, Guidong Miao, Qingyang Wu, Fan Lin, Cuihong You, Shuqi Wang, Jude Juventus Aweya, Hongyu Ma

Abstract

Crab culture has gained prominence in the last decade due to the large global market demand for live crabs and crab products. Portunus sanguinolentus is one of the economically important crab species in the Indo-Pacific region, with distinct differences in growth and size between male and female crabs, thus, leading to huge difference in their market values. The culture of P. sanguinolentus is still in its infancy, with crab supplies heavily dependent on wild catch. In order to unravel the molecular differences between male and female crabs, we generated a comprehensive transcriptomic dataset for P. sanguinolentus by sequencing the gonads of both sexes using the Illumina Hiseq 2500 system. Transcriptomes were assembled using Trinity de novo assembly followed by annotation. This transcriptomic data set for P. sanguinolentus would serve as an important reference data for genomic and genetic studies in this crab and related species.

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