Abstract
Data in experimental biology are frequently marred by outliers and asymmetric distributions. The median, being a robust estimator of central tendency, is less sensitive to outliers than the mean. However, for ranked datasets with an even number of observations, the conventional median-calculated as the average of the two middle values-can introduce bias by implicitly assuming symmetry in the data distribution. This study aims to identify a median estimator that is unbiased. To derive the unbiased median estimator, we minimized the sum of residuals raised to a rational power approaching one. We compared the properties of the unbiased and conventional medians using Poisson-distributed datasets. Random samples were generated with the Mersenne Twister algorithm implemented in IgorPro software (WaveMetrics Inc., Oregon). For odd sample sizes, the unbiased median coincides with the conventional median (the middle value). For even sample sizes, the unbiased median is defined as the value that equalizes the product of distances to data points above and below it-a definition that differs from the conventional median in asymmetric distributions. Although both median estimators tend to underestimate the mean of Poisson-distributed data, the unbiased median is consistently closer to the expected value. Additionally, the unbiased median exhibits lower variance compared to the conventional median. Thus, for even sample sizes, the proposed unbiased median provides a central tendency measure that is unbiased, more accurate, and has reduced variance relative to the conventional median.