Abstract
The role of facultative anaerobic bacteria in colitis remains to be elucidated. We have adapted a mouse model to explore the effect of individual facultative anaerobic bacteria on colitis, focusing on adapting a cocktail of antibiotics and multiple instillations by gavage. Weight, disease activity index, colon length, and histological score are used to assess the severity of colitis. We also describe anaerobic processing protocols of preparing facultative anaerobes. For complete details on the use and execution of this protocol, please refer to Li et al. (2022).1.